ATP Synthase β-Chain Overexpression in SR-BI Knockout Mice Increases HDL Uptake and Reduces Plasma HDL Level
Figure 4
Analysis of DiI-labeled HDL uptake in freshly isolated primary hepatocytes (200x). Primary hepatocytes were pretreated with Ad-ATPase-B1 and Ad-GFP at 30 MOI for 48 h and incubated with 25 μg DiI-HDL for 2 h. (a) Concentration of DiI-HDL and HDL was determined by the dying method with Coomassie brilliant blue. (b) DiI-HDL was detected by fluorescence microscopy (200x) and cellular fluorescent intensities were quantified.
