Functional Analysis of Thyroid Peroxidase Gene Mutations Detected in Patients with Thyroid Dyshormonogenesis

Table 1

Primer sequence used to screen the different exons of TPO gene.

Exons	Primers	Sequence	Amplicon size (bp)
Exon 7	Forward	5′-CTGGAGCTCTGTGAACAAGAA-3′	433
	Reverse	5′-CCCTGGGAATAGGACAAAGAAA-3′
Exon 8	Forward	5′-CCCTACGTAACAAACCTGCAC-3′	474
	Reverse	5′-GGCTGTCAAGGAAGATGCTC-3′
Exon 9	Forward	5′-CGTTGCTTAGAAGGCCTCAG-3′	444
	Reverse	5′-CTTGCAGTGAGCTGAGATCG-3′
Exon 10	Forward	5′-ACAACCTGACCAGGCTTACG-3′	485
	Reverse	5′-CAGGACTCTGCCCTGCTG-3′
Exon 11	Forward	5′-CTGCCCTGAGGGTGTAAGG-3′	446
	Reverse	5′-GAGAGGCTGGCAGCACACAG-3′
Exon 12	Forward	5′-CTATCCCCAGATTGCTCCTG-3′	449
	Reverse	5′-GCTCAGTGAGTGACCACAGC-3′
Exon 13	Forward	5′-GTGTGCTTCGAGGGTCTCTG-3′	485
	Reverse	5′-CCCTAGACCAGGTGGGATG-3′
Exon 14	Forward	5′-CCATGTCCAGAGGAAAGGAG-3′	238
	Reverse	5′-CAGACTCAGGCAGGACAACC-3′