Energy Balance Regulating Neuropeptides Are Expressed through Pregnancy and Regulated by Interleukin-6 Deficiency in Mouse Placenta

Table 2

Primers and probes used for qPCR.

Genes	Primer sequences	GenBank accesion number	Product size (bp)
HPRT	F: 5′-AGCCGACCGGTTCTGTCAT-3′	NM_013556.2	72
	R: 5′-GGTCATAACCTGGTTCATCATCAC-3′
	Pb: 5′-CGACCCTCAGTCCCAGCGTCGTGAT-3′
IL-6	F: 5′-CTATACCACTTCACAAGTCGGAGG-3′	NM_031168.1	77
	R: 5′-TGCACAACTCTTTTCTCATTTCC-3
	Pb: 5′-TTAATTACACATGTTCTCTGGGAAATCG-3′
NPY	F: 5′-ACAGAAAACGCCCCCAGAAC-3′	NM_023456.2	72
	R: 5′-CGGGAGAACAAGTTTCATTTCC-3′
	Pb: 5′-AGGCTTGAAGACCCTTCCATGTGG TGAT-3′
AgRP	F: 5′-ACAACTGCAGACCGAGCAGAA-3′	NM_007427.2	98
	R: 5′-CGACGCGGAGAACGAGACT-3′
	Pb: 5′-CAGAAGGCAGAAGCTTTGGC GG AGGT-3′
CART	F: 5′-CGCAT TCCGATCT ACGAGAAGAA-3′	NM_001081493.2	84
	R: 5′-CCT GGCCCCTT TCCTCACT-3′
	Pb: 5′-CCAAGTCCCCATGTGTGACGCTGGAG-3′.
HPRT	F: 5′-CAGTCCCAGCGTCGTATT-3	NM_013556.2	139
	R: 5′-AGCAAGTCTTTCAGTCCTGTC-3′
POMC	F: 5′-TCCATAGACGTGTGAGCTG-3′	NM_139326	174
	R: 5′-GACGTACTTCCGGGGATTTT-3′