Multiple Components of the VHL Tumor Suppressor Complex Are Frequently Affected by DNA Copy Number Loss in Pheochromocytoma

Figure 2

Disruption of VHL complex component genes. (a) Frequency of DNA copy number alterations and promoter hypermethylation for each component of the VHL elongin BC complex. (b) Venn diagram detailing PCC samples that have copy number loss of VHL complex components either alone or in combination. (c) Incidence of copy number loss of individual components of the VHL complex (RBX1, CUL2, CUL5, TCEB1, TCEB2, and VHL) across a panel of 171 pheochromocytomas. Each row represents an individual complex component affected by copy number loss: RBX1 (light green), CUL5 (yellow), TCEB1 (pink), TCEB2 (dark green), or VHL (aqua). Presence of any alteration in any individual component is shown in red. The bottom row (yellow to red gradient) indicates the gene enrichment score HIF1 gene targets with red bands representing greater enrichment. All samples appear to be enriched for HIF1 targets though not all samples have loss of function events affecting VHL.