The Effects of Lycopene on the Methylation of the GSTP1 Promoter and Global Methylation in Prostatic Cancer Cell Lines PC3 and LNCaP

Figure 2

Lycopene induced expression of GSTP1 in PCa cell lines. qPCR detection of the relative mRNA level of GSTP1 in PC-3 (a) and LNCaP (b) cells with 10 μM of lycopene at 0 days (D0), 7 days (D7), and 14 days (D14) and treated with the DNMT inhibitor AZA and vehicles DMSO and TFH. Western blot analysis of GSTP1 protein in PC-3 (c) and LNCaP (d) cells with different treatments. (e) Immunofluorescence detection of GSTP1 protein expression in PC-3 cells treated by lycopene and AZA. LY, lycopene.

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