Irisin Enhances Osteoblast Differentiation In Vitro
Figure 4
Western blot analysis of Collagen I in total cells lysates (a) and qPCR analysis of ALP in mRNA extracts (b) from osteoblasts treated with conditioned medium (CM) of myoblasts from rest and wheel mice Irisin/FNDC5 neutralizing antibody. The graph (a) shows quantification of OD ratio Collagen I/ -Tubulin as percentage compared to rest CM and is representative for 3 independent experiments. Data is presented as mean ± SEM. Student’s -test was used for single comparisons.
