The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In Vitro

Figure 12

Effects of treatment with 10⁻⁵ M 7-methoxycoumarin or osthole or neither (control) on bone resorption pits formed by rabbit osteoclasts at the 9th day. Bone resorption pits were stained by toluidine blue (a). Number (b), total area (c), and intensity of staining (integrated optical density or IOD) (d) of bone resorption pits were measured by Image-Pro Plus 6.0 software. Data were shown as mean ± SD of triplicate cultures. ^∗𝑃<0.05, ^∗∗𝑃<0.01 versus control; ^#𝑃<0.05, ^##𝑃<0.01 versus 7-methoxycoumarin.

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