The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In Vitro

Figure 5

Time-course changes in mRNA levels of osteogenesis-related genes in rat bone marrow stromal cells (rBMSCs) at 6 h, 12 h, 24 h, 36 h, or 48 h after osteogenic culture treated with 10⁻⁵ M 7-methoxycoumarin or osthole or neither (control). Levels of expression were determined by qRT-PCR and are presented as fold changes relative to the control after being standardized against internal control GAPDH. (a) IGF-1; (b) Runx-2; (c) Osterix; (d) OPG; (e) RANKL; (f) OPG/RANKL. Results are expressed as the mean ± SD, ^∗𝑃<0.05, ^∗∗𝑃<0.01 versus control; ^#𝑃<0.05, ^##𝑃<0.01 versus 7-methoxycoumarin.

(a)

(b)

(c)

(d)

(e)

(f)