The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In Vitro

Table 1

Primer sequences used for qRT-PCR.

Gene	Gene bank number	Primer sequence	Product length (bp)
IGF-1	NM_001082477.2	Forward 5′-TTCAGTTCGTGTGTGGACCAAG-3′	120
		Reverse 5′-GATCACAGCTCCGGAAGCAA-3′
Runx-2	NM_053470.2	Forward 5′-GCACCCAGCCCATAATAGA-3′	165
		Reverse 5′-TTGGAGCAAGGAGAACCC-3′
Osterix	NM_001037632.1	Forward 5′-GCCTACTTACCCGTCTGACTTT-3′	131
		Reverse 5′-GCCCACTATTGCCAACTGC-3′
OPG	NM_012870.2	Forward 5′-TCCTGGCACCTACCTAAA-3′	110
		Reverse 5′-ACACGCATTCATCACTCG-3′
RANKL	NM_057149.1	Forward 5′-CATCGGGTTCCCATAAAG-3′	140
		Reverse 5′-GAAGCAAATGTTGGCGTA-3′
GAPDH	NM_017008.3	Forward 5′-TATCGGACGCCTGGTTAC-3′	140
		Reverse 5′-CTGTGCCGTTGAACTTGC-3′