The Inhibitory Effect of Alisol A 24-Acetate from Alisma canaliculatum on Osteoclastogenesis

Figure 2

Effects of alisol A 24-acetate on osteoclastogenesis. (a) BMMs prepared from bone marrow cells were cultured for 4 days with RANKL (10 ng/mL) and M-CSF (30 ng/mL) in the presence of the indicated concentrations of alisol A 24-acetate. Cells were fixed in 3.7% formalin, permeabilized in 0.1% Triton X-100, and stained for TRAP, a marker enzyme of osteoclasts. (b) TRAP-positive multinuclear cells with three or more nuclei were counted as osteoclasts. ; (). (c) Effect of alisol A 24-acetate on the viability on BMMs was evaluated by CCK-8 assay.

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