Consequence of Menin Deficiency in Mouse Adipocytes Derived by In Vitro Differentiation

Table 1

Differentially regulated genes in Men1-KO versus WT adipocytes derived from mESCs.

Affymetrix probe set ID	Ref seq. transcript ID	Gene symbol	Gene title	Microarray fold change	QPCR fold change
1451634_at	NR_002853	Airn	Antisense Igf2r RNA
1457030_at	NR_028265	Mirg	miRNA containing gene
1438588_at	NM_009538	Plagl1	Pleiomorphic adenoma gene-like 1
1427580_a_at	NR_028261	Rian	RNA imprinted and accumulated in nucleus
1436713_s_at	NR_003633	Meg3	Maternally expressed gene 3
1417836_at	NM_024198	Gpx7	Glutathione peroxidase 7
1421461_at	NM_001122949	Mpl	Myeloproliferative leukemia virus oncogene
1418061_at	NM_013589	Ltbp2	Latent transforming growth factor beta binding protein 2
1428896_at	NM_026840	Pdgfrl	Platelet-derived growth factor receptor-like
1419411_at	NM_009312	Tac2	Tachykinin 2
1423506_a_at	NM_010923	Nnat	Neuronatin
1460412_at	NM_024237	Fbln7	Fibulin 7
1448788_at	NM_010818	Cd200	CD200 antigen
1421074_at	NM_007825	Cyp7b1	Cytochrome P450, family 7, subfamily b, and polypeptide 1
1419197_x_at	NM_032541	Hamp	Hepcidin antimicrobial peptide
1417979_at	NM_022322	Tnmd	Tenomodulin
1425040_at	NM_028593	Cybrd1	Cytochrome b reductase 1
1419292_at	NM_001042615	Htra3	HtrA serine peptidase 3
1435476_a_at	NM_001077189	Fcgr2b	Fc receptor, IgG, low affinity IIb
1416347_at	NM_001168488	Men1	Multiple endocrine neoplasia 1
1450285_at	NM_011667	Ube1y1	Ubiquitin-activating enzyme E1, Chr Y 1
1435064_a_at	NM_020626	Tmem27	Transmembrane protein 27
1448783_at	NM_021291	Slc7a9	Solute carrier family 7 (cationic amino acid transporter, y+ system), member 9
1456601_x_at	NM_007503	Fxyd2	FXYD domain-containing ion transport regulator 2
1424279_at	NM_001111048	Fga	Fibrinogen alpha chain
1418916_a_at	NM_029269	Spp2	Secreted phosphoprotein 2
1449907_at	NM_001163028	Bcmo1	Beta-carotene 15,15′-monooxygenase
1422289_a_at	NM_029636	Ctsq	Cathepsin Q
1418766_s_at	NM_001161355	Timd2	T-cell immunoglobulin and mucin domain-containing 2
1443824_s_at	NM_053070	Car7	Carbonic anhydrase 7
1417761_at	NM_007468	Apoa4	Apolipoprotein A-IV
1418724_at	NM_007686	Cfi	Complement component factor i
1440218_at	NM_001033364	Cdhr2	Cadherin-related family member 2
1452165_at	NM_025532	Prl2b1	Prolactin family 2, subfamily b, member 1
1451513_x_at	NM_009243	Serpina1a	Serine (or cysteine) peptidase inhibitor, clade A, member 1A
1418282_x_at	NM_009244	Serpina1b	Serine (or cysteine) peptidase inhibitor, clade A, member 1B
1424673_at	NM_053165	Clec2h	C-type lectin domain family 2, member h
1429826_at	NM_029355	Prl7b1	Prolactin family 7, subfamily b, member 1
1448572_at	NM_011165	Prl4a1	Prolactin family 4, subfamily a, member 1
1436717_x_at	NM_008221.4	Hbb-y	Hemoglobin Y, beta-like embryonic chain

Validation of top 20 downregulated or upregulated genes () identified in the gene expression microarray analysis. QPCR fold change was normalized to Gapdh. Bold, genes regulated by differential DNA methylation. Italic, genes from the prolactin gene family.