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International Journal of Endocrinology
Volume 2015, Article ID 168047, 11 pages
http://dx.doi.org/10.1155/2015/168047
Research Article

Androgen Receptors Expression in Pituitary of Male Viscacha in relation to Growth and Reproductive Cycle

1Histología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis, Avenida Ejército de los Andes 950, Bloque I, Piso No. 1, 5700 San Luis, Argentina
2Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 5700 San Luis, Argentina

Received 26 November 2014; Revised 6 March 2015; Accepted 8 March 2015

Academic Editor: Małgorzata Kotula-Balak

Copyright © 2015 Verónica Palmira Filippa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this work was to study the androgen receptors (AR) expression in pituitary pars distalis (PD) of male viscachas in relation to growth and reproductive cycle. AR were detected by immunocytochemistry and quantified by image analysis. Pituitary glands from fetus, immature, prepubertal, and adult viscachas during their reproductive cycle were used. In the fetal PD, the immunoreactivity (ir) was mainly cytoplasmic. In immature and prepubertal animals, AR-ir was cytoplasmic (ARc-ir) and nuclear (ARn-ir) in medial region. In adult animals, ARn-ir cells were numerous at caudal end. AR regionalization varied between the PD zones in relation to growth. In immature animals, the ARn-ir increased whereas the cytoplasmic expression decreased in relation to the fetal glands. The percentage of ARc-ir cells increased in prepubertal animals whereas the nuclear AR expression was predominant in adult viscachas. The AR expression changed in adults, showing minimum percentage in the gonadal regression period. The variation of nuclear AR expression was directly related with testosterone concentration. These results demonstrated variations in the immunostaining pattern, regionalization, and number of AR-ir cells throughout development, growth, and reproductive cycle, suggesting the involvement of AR in the regulation of the pituitary activity of male viscacha.