Research Article
Ghrelin’s Effects on Proinflammatory Cytokine Mediated Apoptosis and Their Impact on β-Cell Functionality
Table 1
Primer sets used in real-time quantitative PCR determinations.
| | Gene symbol | Alias/common name | Primer sequence (5′-nyt-3′) | Product size (bp) | Annealing temperature (°C) |
| | HSPA4 | Hsp70, Heat Shock 70 KDa protein 4 (a molecular chaperone involved in ER stress response) | F-AGCAAGCGCTCTCGGTTGCAG |
133 |
60 | | R-AGACAGGACACGGACCCCCG |
| | HSPA5 | Bip, Heat Shock 70 KDa protein 5 (a molecular chaperone involved in ER stress response) | F-TGCTGCTGCCCAACTGGCTG |
160 |
60 | | R-GAACACGCCGACGCAGGAGT |
| | EIF2A | Eukaryotic translation initiation factor 2A | F-ACGCCGCTCTTGACAGTCCG |
152 |
60 | | R-TTGCCCCAGGCAAACAAGGTCC |
| | EIF2AK3 | Eukaryotic translation initiation factor 2 alpha kinase 3 | F-CCCCAACAAGGCCAGCCTGG |
168 |
60 | | R-GGACAGCCAGCCGTGTTCCC |
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