Methylation of Promoter Regions of Genes of the Human Intrauterine Renin Angiotensin System and Their Expression

Figure 1

The proportions of hypermethylated gene alleles for ACE, AGTR1, ATP6AP2, CTSD, and KLK1 and their corresponding mRNA abundances in amnion collected from early gestation (early, ) and normal term pregnancies either by elective caesarean section prior to the onset of labour (C/S, ) or after labour in women who had a spontaneous labour and vaginal delivery (SVB, ). Significant differences were determined with Kruskal-Wallis tests using Dunn’s multiple comparison test, with significant differences denoted by * (adjusted ). Data are presented as Tukey box plots with outliers shown as filled in circles. DNA methylation profiles could not be measured in early gestation for 1/8 ACE and KLK1 samples, from C/S for 1/8 ATP6AP2, 2/8 CTSD, and 5/8 KLK1 samples, and from SVB for 1/8 AGTR1, 4/8 ATP6AP2, 2/8 CTSD, and 5/8 KLK1 samples.