The Blocking on the Cathepsin B and Fibronectin Accumulation in Kidney Glomeruli of Diabetic Rats
Table 3
The values of body weight and kidney weight and ratio of kidney weight/body weight and FN concentrations and cathepsin B activity in homogenates of glomeruli and the content of fibronectin within the glomerulus identified by immunohistochemistry.
Groups
Glomerular homogenates
Immunohistochemistry
Kidney weight/body weight %
Protein/DNA
FN/protein
FN/DNA
Cathepsin B/protein
Cathepsin B/DNA
FN score
μg/μg
ng/μg
ng/μg
μIU/μg
μIU/μg
DM
Mean ± SD
0.60 ± 0.06
23.2 ± 6.50
0.054 ± 0.020
0.95 ± 0.28
1.36 ± 0.37#$
30.60 ± 9.65#
2.33 ± 0.52$
Median (range)
0.6 (0.53–0.68)
23.94 (14.64–32.89)
0.05 (0.029–0.088)
0.89 (0.68–1.48)
1.31 (0.92–1.79)
28.37 (21.09–50.96)
2.00 (2.00-3.00)
EN
Mean ± SD
0.61 ± 0.03
18.1 ± 6.00
0.034 ± 0.018
0.50 ± 0.28
1.5 ± 0.78#$
25.99 ± 16.75#$
2.0 ± 0.82$
Median (range)
0.6 (0.58–0.66)
16.85 (9.10–27.30)
0.031 (0.012–0.069)
0.42 (0.16–1.02)
1.43 (0.58–2.46)
19.14 (12.53–59.90)
2.00 (1.00–3.00)
LOS
Mean ± SD
0.64 ± 0.05$
15.68 ± 5.71
0.056 ± 0.022
0.75 ± 0.30
1.39 ± 0.72#$
21.71 ± 14.32#$
1.60 ± 1.07
Median (range)
0.64 (0.57–0.75)
15.27 (7.75–25.19)
0.055 (0.016–0.09)
0.79 (0.24–1.10)
1.21 (0.49–2.97)
17.98 (10.71–49.64)
2.00 (0.00–3.00)
EN + LOS
Mean ± SD
0.65 ± 0.11
17.65 ± 5.99
0.058 ± 0.016
0.81 ± 0.33
3.11 ± 1.36
51.79 ± 20.37
2.30 ± 0.67$
Median (range)
0.61 (0.57–0.92)
17.73 (10.82–30.62)
0.058 (0.036–0.086)
0.80 (0.50–1.54)
2.78 (1.04–5.32)
53.96 (21.49–80.01)
2.00 (1.00–3.00)
SPIR
Mean ± SD
0.59 ± 0.06
17.31 ± 4.20
0.058 ± 0.022
0.72 ± 0.27
2.58 ± 1.19
44.88 ± 23.13
0.90 ± 0.99
Median (range)
0.59 (0.47–0.67)
16.64 (11.58–24.49)
0.057 (0.021–0.091)
0.70 (0.33–1.13)
2.78 (1.04–4.09)
49.28 (16.59–83.64)
1.00 (0.00–3.00)
DIH
Mean ± SD
0.54 ± 0.05
18.57 ± 3.90
0.062 ± 0.022
0.81 ± 0.38
1.17 ± 0.64#$
22.70 ± 14.97#$
1.70 ± 0.95
Median (range)
0.56 (0.47–0.6)
19.45 (12.91–22.77)
0.054 (0.04–0.097)
0.62 (0.45–1.50)
0.96 (0.52–2.29)
16.02 (7.66–51.97)
1.50 (1.00–4.00)
Results are shown as mean ± SD and median (range). Statistically significant differences between the groups have been shown, the levels of significance in the 95% confidence interval. Statistical significance with the DM group; #statistical significance with the EN + LOS group; $statistical significance with the SPIR group; statistical significance with the DIH group. Kidney weight/body weight value: DM versus DIH = 0.01; DIH versus EN = 0.01; DIH versus LOS = 0.0001; DIH versus EN + LOS = 0.01; DIH versus SPIR = 0.04; SPIR versus LOS = 0.03. Protein/DNA value: DM versus LOS = 0.01; DM versus SPIR = 0.045. Cathepsin B/protein value: DM versus EN + LOS = 0.001; DM versus SPIR = 0.02; EN + LOS versus EN = 0.002; EN + LOS versus LOS = 0.001; EN + LOS versus DIH = 0.0004; SPIR versus EN = 0.03; SPIR versus LOS = 0.02; SPIR versus DIH = 0.009. Cathepsin B/DNA value: DM versus EN + LOS = 0.04; EN + LOS versus EN = 0.01; EN + LOS versus LOS = 0.01; EN + LOS versus DIH = 0.01; SPIR versus EN = 0.05; SPIR versus LOS = 0.02; SPIR versus DIH = 0.03. FN by immunohistochemistry -value: DM versus SPIR = 0.003; SPIR versus EN = 0.02; SPIR versus EN + LOS = 0.003.
