Effect of Advanced Glycation End Products on Human Thyroglobulin’s Antigenicity as Identified by the Use of Sera from Patients with Hashimoto’s Thyroiditis and Gestational Diabetes Mellitus

Table 1

Effect of modifications at the in vitro glycation method on Tg’s AGE content.

	D-glucose pH adjustment^a
	No (pH = 1)				Yes (pH = 7.4)
D-glucose concentration	0.11 M	0.05 M	0.03 M	0 M	0.03 M	0.004 M
Units AGE/mg Tg^b	49	31	N.D.	0.78	13	N.D.

D-glucose-6-phosphate solution pH was either adjusted or not to 7.4 prior to its mixing with Tg.
^bUnits AGE/mg Tg were determined by competitive ELISA.