Methylation of the Corticotropin Releasing Hormone Gene Promoter in BeWo Cells: Relationship to Gene Activity

Figure 1

CRH mRNA relative abundance in BeWo cells treated with 8-Br-cAMP (cAMP, 2.5 × 10⁻⁴ mol L⁻¹) and 5-AZA-dC (AZA, 5 × 10⁻⁶ mol L⁻¹) for 24 h, 48 h, and 72 h. The average ± SEM of results of four independent experiments is shown. Significant pairwise differences (-test) between vehicle-treated and 5-AZA-dC-treated cultures are indicated. Significant pairwise differences involving 8-Br-cAMP-treated cultures and 8-Br-cAMP + 5-AZA-dC-treated cultures are described in the Results. ^∗Significantly higher than 0 h (, one-sample, one-sided -test).