Paraoxonase 2 Induces a Phenotypic Switch in Macrophage Polarization Favoring an M2 Anti-Inflammatory State

Figure 1

MPM from PON2KO display increased proinflammatory activation. MPM were harvested from 3-month-old control (C57BL/6) male mice and from age matched PON2KO mice. The cells were stimulated toward M1 activation with LPS (100 ng/mL) and IFNγ (20 ng/mL) for 12 hours. Secretion of TNFα and IL-6 to the medium was measured under basal (a) or M1 stimulation (c) conditions. TNFα and IL-6 mRNA expression, analyzed by quantitative PCR and normalized to GAPDH, were measured in the cells under basal (b) or M1 stimulation (d) conditions. Arginase II mRNA expression was also measured under basal or M1 stimulation conditions (e). Results are expressed as mean ± SD (), , PON2KO versus control MPM.

(a) Unstimulated MPM

(b) Unstimulated MPM

(c) M1 stimulated MPM

(d) M1 stimulated MPM

(e) Arginase II mRNA expression