Paraoxonase 2 Induces a Phenotypic Switch in Macrophage Polarization Favoring an M2 Anti-Inflammatory State

Figure 2

MPM from PON2KO display reduced anti-inflammatory activation. MPM were harvested from 3-month-old control (C57BL/6) male mice and from age matched PON2KO mice. The cells were stimulated toward M2 activation with IL-4 (20 ng/mL) for 12 hours. Secretion of IL-10 to the medium was measured under basal (a) or M2 stimulation (c) conditions. IL-10 mRNA expression, analyzed by quantitative PCR and normalized to GAPDH, was measured in the cells under basal (b) or M2 stimulation (d) conditions. Arginase I mRNA expression was also measured under basal or M2 stimulating conditions (e). Results are expressed as mean ± SD (), , PON2KO MPM versus control MPM.

(a) Unstimulated MPM

(b) Unstimulated MPM

(c) M2 stimulated MPM

(d) M2 stimulated MPM

(e) Arginase I expression