Genome-Wide DNA Methylation in Mixed Ancestry Individuals with Diabetes and Prediabetes from South Africa

Figure 1

MeDIP-enriched regions (peaks) identified in all the samples. Total sites are the sum of the peak numbers for subjects with diabetes, subjects with prediabetes, and controls and include all the peaks that may be common/redundant across the conditions, as well as the peaks unique to each condition. Distributions of peaks in intergenic (I), gene body (GB), and promoter (P) regions are shown. Statistically significant MeDIP-enriched regions (peaks) were detected by MACS2 and identified by comparison to a Poisson background model, using a q-value threshold of 10⁻². MAnorm was used to calculate differentially methylated regions with statistical significance. The differentially methylated regions located within gene promoters [TSS − 2000 bp; TSS + 2000 bp] are selected and provided. By peak number, there are more hypermethylated peaks in individuals with diabetes (82%) than in the control subjects.