Interaction between Angiotensin II and Insulin/IGF-1 Exerted a Synergistic Stimulatory Effect on ERK1/2 Activation in Adrenocortical Carcinoma H295R Cells

Figure 1

Ang II caused dose-dependent activation of ERK1/2 in H295R cells. Serum-starved H295R cells were treated with Ang II at different doses (1, 10, 100, and 1000 nM) for 5 min, then lysed in Laemmli sample buffer, and analyzed by SDS-PAGE using antibody against phospho-ERK1/2 (Thr202/Tyr204) and phospho-AKT (Ser473). The bolt was reprobed with total ERK1/2 and total AKT antibody loading controls.