Interaction between Angiotensin II and Insulin/IGF-1 Exerted a Synergistic Stimulatory Effect on ERK1/2 Activation in Adrenocortical Carcinoma H295R Cells

Figure 4

Involvement of MEK in agonists-stimulated phospho-ERK1/2 and phospho-AKT in H295R cells. (a) Effects of U0126, a MEK1/2 inhibitor, on Ang II-induced ERK1/2 and AKT phosphorylation. H295R cells were treated with increasing concentrations of U0126 for 30 min and then stimulated with Ang II (100 nM) for 5 min. (b) Quantitative determination of the effects of U0126 on ERK1/2 and AKT phosphorylation by Ang II from panels (a). (c and d) After incubation with U0126 (10 μM) for 30 min, H295R cells were pretreated with Ang II for another 30 min and then stimulated with insulin/IGF-1 for 5 min. (c) Insulin (100 nM); (d) IGF-1 (10 nM).

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