Interaction between Angiotensin II and Insulin/IGF-1 Exerted a Synergistic Stimulatory Effect on ERK1/2 Activation in Adrenocortical Carcinoma H295R Cells

Figure 5

Involvement of PKC in agonists-stimulated phospho-ERK1/2 and phospho-AKT in H295R cells. (a) Effects of Gö6983, a PKC inhibitor, on Ang II-induced ERK1/2 and AKT phosphorylation. H295R cells were treated with increasing concentrations of Gö6983 for 30 min and then stimulated with Ang II (100 nM) for 5 min. (b) Quantitative determination of the effects of Gö6983 on Ang II-induced ERK1/2 and AKT phosphorylation from panels (a). (c and d) After incubation with Gö6983 (5 μM) for 30 min, H295R cells were pretreated with Ang for another 30 min and then stimulated with insulin/IGF-1 for 5 min. (c) Insulin (100 nM); (d) IGF-1 (10 nM).

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