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International Journal of Endocrinology
Volume 2016 (2016), Article ID 3797139, 9 pages
http://dx.doi.org/10.1155/2016/3797139
Research Article

The Influence of Alcohol Consumption in Conjunction with Sex Hormone Deficiency on Ca/P Ratio in Rats

1Institute of Science and Technology, Department of Biosciences and Diagnostics, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo 777, 12245-000 São José dos Campos, SP, Brazil
2Institute of Environmental Science, Chemical and Pharmaceutical, Department of Mathematical and Earth Sciences, Federal University of São Paulo (UNIFESP), Rua Prof. Artur Riedel 275, 09972-270 Diadema, SP, Brazil
3Institute of Research and Development, University of Vale do Paraíba (UNIVAP), Laboratory of Biomedical Vibrational Spectroscopy, Avenida Shishima Hifumi 2911, 12244-000 São José dos Campos, SP, Brazil
4Institute of Science and Technology, Department of Dental Materials and Prosthesis, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Francisco José Longo 777, 12245-000 São José dos Campos, SP, Brazil
5Department of Preventive and Community Dentistry, The University of Iowa College of Dentistry and Dental Clinics, N337-1 Dental Science N, 52242 Iowa City, IA, USA

Received 12 October 2015; Revised 16 February 2016; Accepted 22 February 2016

Academic Editor: Marek Bolanowski

Copyright © 2016 Karina Bortolin Lodi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Deficiency of sex hormones and excessive alcohol consumption are factors that have been related to alterations in the pattern of bone mineralization and osteoporosis. The aim of this study was to evaluate possible alterations in the calcium/phosphorus (Ca/P) ratio in the femur of rats subjected to sex hormone deficiency and/or alcohol consumption. Methods. Female and male Wistar rats () were divided into ovariectomized (Ovx), orchiectomized (Orx), or sham-operated groups and subdivided according to diet: alcoholic diet (20% alcohol solution), isocaloric diet, and ad libitum diet. The diets were administered for 8 weeks. The Ca/P ratio in the femur was analyzed by energy dispersive micro-X-ray spectrometer (μEDX). Results. Consumption of alcohol reduced the Ca/P ratio in both females and males. The isocaloric diet reduced the Ca/P ratio in females. In groups with the ad libitum diet, the deficiency of sex hormones did not change the Ca/P ratio in females or males. However, the combination of sex hormone deficiency and alcoholic diet presented the lowest values for the Ca/P ratio in both females and males. Conclusions. There was a reduced Ca/P ratio in the femur of rats that consumed alcohol, which was exacerbated when combined with a deficiency of sex hormones.