Ghrelin Protects against Dexamethasone-Induced INS-1 Cell Apoptosis via ERK and p38MAPK Signaling

Figure 1

Effects of ghrelin and dexamethasone on INS-1 cell viability. (a, b) INS-1 cells were seeded at a density of 1 × 10⁴ cells/well in 96-well plates and treated with 0.001–1 μM ghrelin or dexamethasone for 48 h. Cell viability was assessed using the MTS assay. (c) Ghrelin protects INS-1 cells from dexamethasone-induced cell apoptosis. INS-1 cells were incubated in complete medium (control), 0.1 μM dexamethasone, 0.1 μM ghrelin, and 0.1 μM dexamethasone + 0.1 μM ghrelin for 48 h. The Promega cell proliferation assay was used to determine the viability of INS-1 cells. The data are presented as the mean ± SD. versus control group; versus dexamethasone group.

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