Ghrelin Protects against Dexamethasone-Induced INS-1 Cell Apoptosis via ERK and p38MAPK Signaling

Figure 5

The effect of MAPK on dexamethasone-induced INS-1 cell apoptosis. (a) The effect of dexamethasone and ghrelin on MAPK expression in INS-1 cells. The expression of phospho-MAPK and MAPK in treated INS-1 cells was detected by western blot analysis. (b, c, d) Graphs indicate the quantitative analyses of pMAPK and MAPK. (e) The effect of ERK and p38MAPK inhibitors on INS-1 cell apoptosis after dexamethasone treatment. The ERK inhibitor U0126 (10 μM) and p38MAPK inhibitor SB203580 (10 μM) were added 15 min before dexamethasone (0.1 μM) treatment. Cells were then stained with annexin V-FITC and PI and sorted by fluorescence flow cytometry after dexamethasone treatment for 48 h. Graphs represent the percentage of INS-1 cell apoptosis. Data are presented as the mean ± SD. , versus control group; versus dexamethasone group.

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