Table of Contents Author Guidelines Submit a Manuscript
International Journal of Endocrinology
Volume 2016, Article ID 4513051, 11 pages
http://dx.doi.org/10.1155/2016/4513051
Research Article

Ghrelin Protects against Dexamethasone-Induced INS-1 Cell Apoptosis via ERK and p38MAPK Signaling

1Hepatobiliary Surgery Department and Unit of Organ Transplantation, First Hospital of China Medical University, Shenyang 110001, China
2Hepatobiliary Surgery Department, Chifeng Municipal Hospital, Chifeng 024000, China
3Department of General Surgery, Fourth Hospital of China Medical University, Shenyang 110032, China

Received 26 November 2015; Revised 10 March 2016; Accepted 23 March 2016

Academic Editor: Marco Bugliani

Copyright © 2016 Chengshuo Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. H. Schäcke, W.-D. Döcke, and K. Asadullah, “Mechanisms involved in the side effects of glucocorticoids,” Pharmacology and Therapeutics, vol. 96, no. 1, pp. 23–43, 2002. View at Publisher · View at Google Scholar · View at Scopus
  2. F. Ranta, D. Avram, S. Berchtold et al., “Dexamethasone induces cell death in insulin-secreting cells, an effect reversed by exendin-4,” Diabetes, vol. 55, no. 5, pp. 1380–1390, 2006. View at Publisher · View at Google Scholar · View at Scopus
  3. D. Hamamdzic, E. Duzic, J. D. Sherlock, and S. M. Lanier, “Regulation of α2-adrenergic receptor expression and signaling in pancreatic β-cells,” American Journal of Physiology—Endocrinology and Metabolism, vol. 269, no. 1, pp. E162–E171, 1995. View at Google Scholar · View at Scopus
  4. S. Ullrich, S. Berchtold, F. Ranta et al., “Serum- and glucocorticoid-inducible kinase 1 (SGK1) mediates glucocorticoid-induced inhibition of insulin secretion,” Diabetes, vol. 54, no. 4, pp. 1090–1099, 2005. View at Publisher · View at Google Scholar · View at Scopus
  5. S. Gremlich, R. Roduit, and B. Thorens, “Dexamethasone induces posttranslational degradation of GLUT2 and inhibition of insulin secretion in isolated pancreatic β cells. Comparison with the effects of fatty acids,” Journal of Biological Chemistry, vol. 272, no. 6, pp. 3216–3222, 1997. View at Publisher · View at Google Scholar · View at Scopus
  6. A. J. Weinhaus, N. V. Bhagroo, T. C. Brelje, and R. L. Sorenson, “Dexamethasone counteracts the effect of prolactin on islet function: implications for islet regulation in late pregnancy,” Endocrinology, vol. 141, no. 4, pp. 1384–1393, 2000. View at Publisher · View at Google Scholar · View at Scopus
  7. M. Kojima, H. Hosoda, Y. Date, M. Nakazato, H. Matsuo, and K. Kangawa, “Ghrelin is a growth-hormone-releasing acylated peptide from stomach,” Nature, vol. 402, no. 6762, pp. 656–660, 1999. View at Publisher · View at Google Scholar · View at Scopus
  8. A. M. Wren, C. J. Small, C. R. Abbott et al., “Ghrelin causes hyperphagia and obesity in rats,” Diabetes, vol. 50, no. 7–12, pp. 2540–2547, 2001. View at Publisher · View at Google Scholar · View at Scopus
  9. C.-Y. Chen, A. Inui, A. Asakawa et al., “Des-acyl ghrelin acts by CRF type 2 receptors to disrupt fasted stomach motility in conscious rats,” Gastroenterology, vol. 129, no. 1, pp. 8–25, 2005. View at Publisher · View at Google Scholar · View at Scopus
  10. K. Toshinai, H. Yamaguchi, Y. Sun et al., “Des-acyl ghrelin induces food intake by a mechanism independent of the growth hormone secretagogue receptor,” Endocrinology, vol. 147, no. 5, pp. 2306–2314, 2006. View at Publisher · View at Google Scholar · View at Scopus
  11. W. Zhang, B. Chai, J.-Y. Li, H. Wang, and M. W. Mulholland, “Effect of des-acyl ghrelin on adiposity and glucose metabolism,” Endocrinology, vol. 149, no. 9, pp. 4710–4716, 2008. View at Publisher · View at Google Scholar · View at Scopus
  12. J.-C. Chuang, I. Sakata, D. Kohno et al., “Ghrelin directly stimulates glucagon secretion from pancreatic α-cells,” Molecular Endocrinology, vol. 25, no. 9, pp. 1600–1611, 2011. View at Publisher · View at Google Scholar · View at Scopus
  13. T. Yada, B. Damdindorj, R. S. Rita et al., “Ghrelin signalling in β-cells regulates insulin secretion and blood glucose,” Diabetes, Obesity and Metabolism, vol. 16, supplement 1, pp. 111–117, 2014. View at Publisher · View at Google Scholar · View at Scopus
  14. R. Granata, A. Baragli, F. Settanni, F. Scarlatti, and E. Ghigo, “Unraveling the role of the ghrelin gene peptides in the endocrine pancreas,” Journal of Molecular Endocrinology, vol. 45, no. 3, pp. 107–118, 2010. View at Publisher · View at Google Scholar · View at Scopus
  15. R. Granata, F. Settanni, L. Biancone et al., “Acylated and unacylated ghrelin promote proliferation and inhibit apoptosis of pancreatic β-cells and human islets: involvement of 3′,5′-cyclic adenosine monophosphate/protein kinase A, extracellular signal-regulated kinase 1/2, and phosphatidyl inositol 3-kinase/Akt signaling,” Endocrinology, vol. 148, no. 2, pp. 512–529, 2007. View at Publisher · View at Google Scholar · View at Scopus
  16. M. Bando, H. Iwakura, H. Ariyasu et al., “Overexpression of intraislet ghrelin enhances β-cell proliferation after streptozotocin-induced β-cell injury in mice,” American Journal of Physiology-Endocrinology and Metabolism, vol. 305, no. 1, pp. E140–E148, 2013. View at Publisher · View at Google Scholar · View at Scopus
  17. M. Asfari, D. Janjic, P. Meda, G. Li, P. A. Halban, and C. B. Wollheim, “Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines,” Endocrinology, vol. 130, no. 1, pp. 167–178, 1992. View at Google Scholar · View at Scopus
  18. A. Salic and T. J. Mitchison, “A chemical method for fast and sensitive detection of DNA synthesis in vivo,” Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 7, pp. 2415–2420, 2008. View at Publisher · View at Google Scholar · View at Scopus
  19. J. M. Kyriakis and J. Avruch, “Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation,” Physiological Reviews, vol. 81, no. 2, pp. 807–869, 2001. View at Google Scholar · View at Scopus
  20. B. Li, M. Zeng, W. He et al., “Ghrelin protects alveolar macrophages against lipopolysaccharide-induced apoptosis through growth hormone secretagogue receptor 1a-dependent c-jun N-terminal kinase and Wnt/β-catenin signaling and suppresses lung inflammation,” Endocrinology, vol. 156, no. 1, pp. 203–217, 2015. View at Publisher · View at Google Scholar · View at Scopus
  21. Q.-H. Liang, Y. Liu, S.-S. Wu, R.-R. Cui, L.-Q. Yuan, and E.-Y. Liao, “Ghrelin inhibits the apoptosis of MC3T3-E1 cells through ERK and AKT signaling pathway,” Toxicology and Applied Pharmacology, vol. 272, no. 3, pp. 591–597, 2013. View at Publisher · View at Google Scholar · View at Scopus
  22. J. Y. Lee, T. H. Oh, and T. Y. Yune, “Ghrelin inhibits hydrogen peroxide-induced apoptotic cell death of oligodendrocytes via ERK and p38MAPK signaling,” Endocrinology, vol. 152, no. 6, pp. 2377–2386, 2011. View at Publisher · View at Google Scholar · View at Scopus
  23. W.-J. Fan, S. Genade, A. Genis, B. Huisamen, and A. Lochner, “Dexamethasone-induced cardioprotection: a role for the phosphatase MKP-1?” Life Sciences, vol. 84, no. 23-24, pp. 838–846, 2009. View at Publisher · View at Google Scholar · View at Scopus
  24. S. Yu and Y. Li, “Dexamethasone inhibits podocyte apoptosis by stabilizing the PI3K/Akt signal pathway,” BioMed Research International, vol. 2013, Article ID 326986, 9 pages, 2013. View at Publisher · View at Google Scholar · View at Scopus
  25. Y.-N. Zhao, X. Guo, Z.-G. Ma, L. Gu, J. Ge, and Q. Li, “Pro-apoptotic protein BIM in apoptosis of glucocorticoid-sensitive and -resistant acute lymphoblastic leukemia CEM cells,” Medical Oncology, vol. 28, no. 4, pp. 1609–1617, 2011. View at Publisher · View at Google Scholar · View at Scopus
  26. L. P. Roma, C. A. M. Oliveira, E. M. Carneiro, G. G. Albuquerque, A. C. Boschero, and K. L. A. Souza, “N-acetylcysteine protects pancreatic islet against glucocorticoid toxicity,” Redox Report, vol. 16, no. 4, pp. 173–180, 2011. View at Publisher · View at Google Scholar · View at Scopus
  27. B. D. Obay, E. Taşdemir, C. Tümer, H. M. Bilgin, and M. Atmaca, “Dose dependent effects of ghrelin on pentylenetetrazole-induced oxidative stress in a rat seizure model,” Peptides, vol. 29, no. 3, pp. 448–455, 2008. View at Publisher · View at Google Scholar · View at Scopus
  28. S. Dinicola, V. Morini, P. Coluccia et al., “Nicotine increases survival in human colon cancer cells treated with chemotherapeutic drugs,” Toxicology in Vitro, vol. 27, no. 8, pp. 2256–2263, 2013. View at Publisher · View at Google Scholar · View at Scopus
  29. M. Riopel, M. Krishnamurthy, J. Li, S. Liu, A. Leask, and R. Wang, “Conditional β1-integrin-deficient mice display impaired pancreatic β cell function,” Journal of Pathology, vol. 224, no. 1, pp. 45–55, 2011. View at Publisher · View at Google Scholar · View at Scopus
  30. A. Rak-Mardyla and E. L. Gregoraszczuk, “ERK 1/2 and PI-3 kinase pathways as a potential mechanism of ghrelin action on cell proliferation and apoptosis in the porcine ovarian follicular cells,” Journal of Physiology and Pharmacology, vol. 61, no. 4, pp. 451–458, 2010. View at Google Scholar · View at Scopus
  31. J. Y. Lee, H. Chung, Y. S. Yoo et al., “Inhibition of apoptotic cell death by ghrelin improves functional recovery after spinal cord injury,” Endocrinology, vol. 151, no. 8, pp. 3815–3826, 2010. View at Publisher · View at Google Scholar · View at Scopus
  32. T. H. Holmström, I. Schmitz, T. S. Söderström et al., “MAPK/ERK signaling in activated T cells inhibits CD95/Fas-mediated apoptosis downstream of DISC assembly,” The EMBO Journal, vol. 19, no. 20, pp. 5418–5428, 2000. View at Publisher · View at Google Scholar · View at Scopus
  33. H. Chung, E. Kim, D. H. Lee et al., “Ghrelin inhibits apoptosis in hypothalamic neuronal cells during oxygen-glucose deprivation,” Endocrinology, vol. 148, no. 1, pp. 148–159, 2007. View at Publisher · View at Google Scholar · View at Scopus
  34. H. Wang, D. Jiang, J. Liu et al., “Compound K induces apoptosis of bladder cancer T24 cells via reactive oxygen species-mediated p38 MAPK Pathway,” Cancer Biotherapy and Radiopharmaceuticals, vol. 28, no. 8, pp. 607–614, 2013. View at Publisher · View at Google Scholar · View at Scopus
  35. T. D. Müller, R. Nogueiras, M. L. Andermann et al., “Ghrelin,” Molecular Metabolism, vol. 4, no. 6, pp. 437–460, 2015. View at Publisher · View at Google Scholar
  36. Y. Sun, M. Asnicar, P. K. Saha, L. Chan, and R. G. Smith, “Ablation of ghrelin improves the diabetic but not obese phenotype of ob/ob mice,” Cell Metabolism, vol. 3, no. 5, pp. 379–386, 2006. View at Publisher · View at Google Scholar · View at Scopus
  37. B. P. Barnett, Y. Hwang, M. S. Taylor et al., “Glucose and weight control in mice with a designed ghrelin O-acyltransferase inhibitor,” Science, vol. 330, no. 6011, pp. 1689–1692, 2010. View at Publisher · View at Google Scholar · View at Scopus
  38. E. Adeghate and A. S. Ponery, “Ghrelin stimulates insulin secretion from the pancreas of normal and diabetic rats,” Journal of Neuroendocrinology, vol. 14, no. 7, pp. 555–560, 2002. View at Publisher · View at Google Scholar · View at Scopus
  39. R. Granata, M. Volante, F. Settanni et al., “Unacylated ghrelin and obestatin increase islet cell mass and prevent diabetes in streptozotocin-treated newborn rats,” Journal of Molecular Endocrinology, vol. 45, no. 1, pp. 9–17, 2010. View at Publisher · View at Google Scholar · View at Scopus