Tetrahydro-iso-alpha Acids Antagonize Estrogen Receptor Alpha Activity in MCF-7 Breast Cancer Cells

Figure 11

Effect of THIAA on LxxLL motif recruitment assessed by TR-FRET (a) and ELISA (b). For TR-FRET experiments, recombinant ERα was incubated in the absence (control) or presence of E₂ at 10⁻⁶M with or without THIAA at 0.001% or an LxxLL competitor peptide at 10⁻⁴M. Fluorescence emission was measured at 488 nm and 518 nm under a 332 nm excitation wavelength. Data refer to the mean value ± SD of Relative Fluorescence Unit (RLU) ratio at 518 nm and 488 nm of an experiment performed twice in triplicate. For ELISA, cell extracts of MCF-7 treated or not with E₂ at 10⁻⁹M (30 min) were incubated with or without THIAA at 0.001% or an LxxLL competitor peptide at 10⁻⁴M. Cell extracts where then submitted to this ELISA in which the capture element is an LxxLL peptide. Data refer to an experiment performed three times.

(a)

(b)