Research Article
Tetrahydro-iso-alpha Acids Antagonize Estrogen Receptor Alpha Activity in MCF-7 Breast Cancer Cells
Figure 8
Effect of THIAA on PgR expression in steroid-free culture medium by western blot. MCF-7 cells were incubated for 24 h in the absence (control) or presence of E2 at 10−9 M with or without THIAA at 0.001%. Immunoblots and optical density (OD) analyses of PgRA and PgRB refer to an experiment performed independently twice. ODs of both PgR isoforms are normalized by ODs of actin and expressed as percentage of control.