Research Article
Tetrahydro-iso-alpha Acids Antagonize Estrogen Receptor Alpha Activity in MCF-7 Breast Cancer Cells
Figure 9
Effect of THIAA on ERα protein level in steroid-free culture medium by immunofluorescence (a) and western blot (b). MCF-7 cells were incubated for 24 h in the absence (control) or presence of E2 at 10−9 M with or without THIAA at 0.001%. Immunofluorescence, immunoblots, and optical density (OD) analyses of ERα refer to an experiment performed independently twice. ODs of ERα are normalized by ODs of actin and expressed as percentage of control.
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