Functional Characterization of Preadipocytes Derived from Human Periaortic Adipose Tissue

Figure 3

PAT expressed proteins involved in thermogenesis. (a) Samples from SAT and PAT were obtained and immediately frozen in liquid nitrogen. mRNA was extracted and the detection of PGC-1α and UCP-1 was performed by qPCR. (b) Precursor adipose cells (PAC) from SAT and PAT were induced to differentiate into mature adipocytes (MAT), and proteins were extracted to quantify the levels of PGC-1α, UCP-1, CITED1, and TFAM by Western blotting. (c) Relative intensity of the protein bands was determined by densitometry. Data were normalized to the housekeeping protein GAPDH and expressed as means ± SD (). , in three different studies, indicates a statistically significant difference in protein levels in precursor adipose cells (SAT versus PAT) and mature adipocyte cells (SAT versus PAT). indicates the difference between PAT and SAT both in precursor adipocytes and mature adipocytes. indicates the difference between PAT and SAT in mature adipocytes.

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