Functional Characterization of Preadipocytes Derived from Human Periaortic Adipose Tissue

Figure 4

PGC-1α expression and the relationship between PAT and SAT. (a) Adipocyte precursor cells were isolated from PAT and SAT, induced to proliferate, fixed in 10% formaldehyde, and stained for the presence of PGC-1α by immunofluorescence. The fluorescent intensity was quantified with ImageJ software. Data are expressed as means ± SD. indicates the differences in the fluorescent intensity between SAT and PAT. Data correspond to the three different studies. (b) The scatter plot shows the relationship for the PGC-1α level in precursor cells from PAT and SAT. Each data point represents a patient. The y-axis (Y) shows the trend toward PAT expression, and the x-axis (X) shows the trend toward SAT expression (). The bars indicate intensity levels as determined by densitometry of the bands between SAT and PAT from each patient using paired Student’s t-test of Western blot analysis of PGC-1α. indicates significative differences in PGC-1α protein expression between SAT and PAT.

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