Muscarinic Receptors Types 1 and 2 in the Preoptic-Anterior Hypothalamic Areas Regulate Ovulation Unequally in the Rat Oestrous Cycle

Figure 2

Schematic representation of the experiments A and B. Experiment A: experimental design for unilaterally microinjecting drugs (PZP, MTC, or vehicle) on the left or right side of the POA-AHA region on the day of oestrous, dioestrous-1, or dioestrous-2. Experiment B: experimental design for re-establishing the hypothalamic signal through the replacement of LH-releasing hormone (LHRH) or oestradiol benzoate (EB). All animals were sacrificed on the predicted day of oestrous.