International Journal of Endocrinology / 2017 / Article / Fig 4

Research Article

Exploring the Role of Estrogens in Lizard Spermatogenesis through the Study of Clomiphene and FSH Effects

Figure 4

Immunohistochemistry with ERα (a–d) or VTG (bottom panel, e–f) antibodies in the liver. (a) Untreated males: no immunoreactivity is detected to anti-ERα antibody; the box (A) shows the negative control of reaction, performed lacking the antibody. (b) Clomiphene-treated and (c) FSH-treated samples: ir-ERα is evident in cells. (d) FSH-Clomiphene: no immunoreactivity is detected as in untreated samples (a). (e) Untreated males: no immunoreactivity is detected to anti-VTG antibody. (f) Clomiphene-treated and (g) FSH-treated samples: ir-VTG is evident in the cytoplasm cells. (h) FSH-Clomiphene: no immunoreactivity is detected, as in untreated samples (e). In (e), (f), (g), and (h), cell nuclei were counterstained with Mayer’s haemalum. The bar is 30 μm.
(a)
(b)
(c)
(d)
(e)
(f)
(g)
(h)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.