Crosstalk between Aryl Hydrocarbon Receptor and Glucocorticoid Receptor in Human Retinal Pigment Epithelial Cells

Figure 5

The interaction between AHR and GR induced by TCDD and Dex. A coimmunoprecipitation assay was carried out using ARPE-19 cells transfected with GR expression plasmid. At 72 h after transfection, cells were treated with TCDD (30 nM), Dex (100 nM), or TCDD (30 nM) + Dex (100 nM) for 30 min. Then, cell lysates were prepared in RIPA buffer. Coimmunoprecipitation assay was performed using normal mouse IgG or anti-GR antibody. Immunoblotting was performed using an anti-AHR antibody or anti-GR antibody. The relative protein levels of AHR were quantified by scanning densitometry and normalized to GR. The images shown are representatives of three independent experiments that showed consistent results, and the relative protein values are expressed as mean ± SD for three independent experiments. and .

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