PDE5 Overexpression in Well-Differentiated Thyroid Carcinomas Is Associated with Lymph Node Metastasis

Figure 1

Upregulation of PDE5 expression in well-differentiated thyroid carcinoma (WDTC). (a, b, c) Expression of PDE5 in WDTCs as determined by immunohistochemistry. Strong staining (A1, ×10; A2, ×40) and negative staining (B1, ×10; B2, ×40) can be observed. A WDTC sample (C1, ×10) with strong PDE5 staining in the carcinoma tissues (C2, ×40) and normal thyroid follicular epithelial cells with weak staining (C3, ×40). (d and e) Western blots show the expression levels of the PDE5 protein in four WDTC tumor tissues (T) and their paired adjacent noncancerous tissues (ANT); β-Actin was used as the loading control. (f) Real-time qPCR analysis results show the expression levels of PDE5 mRNA in WDTC tumor tissues relative to their paired adjacent noncancerous tissues (T/ANT). Values are given as the ratio of PDE5 expression to GAPDH expression (, , and ).

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