Chronic Hyperinsulinaemic Hypoglycaemia in Rats Is Accompanied by Increased Body Weight, Hyperleptinaemia, and Decreased Neuronal Glucose Transporter Levels in the Brain

Table 2

Antibodies used for western blotting.

Transporter	Primary antibody; (cat. number)	Company	Working; dilution	Size of band(s); detected (kDa)	Blocking peptide; (cat. number)	Host species of; primary antibody	Secondary; antibody dilution^d
GLUT1	07-1401	MLS^a	1:8000	43	N/A	Rabbit	1:3000
GLUT3	ab15311	Abcam^b	1:400	38, 45, and 50	N/A	Rabbit	1:1500
SGLT1	ab14686	Abcam	1:1000	30, (42)	ab190911	Rabbit	1:2000
MCT1	AB3540P	MLS^a	1:5000	40	N/A	Rabbit	1:4000
Actin	Ab1801	Abcam	1:1000	10, 25, 30, and 42^c	ab13771	Rabbit	1:2000

N/A, not applicable as the corresponding blocking peptide was not commercially available; instead, one was custom synthesized (CASLO ApS, Kgs. Lyngby, Denmark) according to the peptide sequence supplied by the manufacturer of the primary antibody. All custom-synthesized blocking peptides were supplied at >95% purity. ^aMerck Life Science A/S, Hellerup, Denmark. ^bAbcam plc., Cambridge, UK. ^cTriple band. ^dThe same secondary goat anti-rabbit (#170-6515, Bio-Rad Laboratories, Copenhagen, Denmark) was used for all primary antibodies.