Cholesterol Synthesis Increased in the MMI-Induced Subclinical Hypothyroidism Mice Model

Figure 3

The phosphorylation of HMGCR and AMPK decreased in SCH mice. (a) The expression levels of p-HMGCR, t-HMGCR, p-AMPK, and t-AMPK in SCH and control mice were analyzed using western immunoblotting analysis. The same membranes were reprobed with anti-β-actin antibody to confirm equal loading of proteins for each sample. Representative results from three independent experiments are shown. (b) The ratios of p-HMGCR/HMGCR in the livers of control mice and SCH mice were assessed by semiquantitative analysis. (c) Hepatic HMGCR activity was measured in the presence of NaF. (d) The ratios of p-AMPK/AMPK in the livers of control mice and SCH mice. (e) The expression of precursor and mature SREBP-2 in SCH and control mice. Data are representative of three independent experiments. and versus control mice.

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