Variations in the 3′UTR of the CYP21A2 Gene in Heterozygous Females with Hyperandrogenaemia

Figure 1

CYP21A2 mRNA secondary structure prediction. (a) and (b) predicted CYP21A2 mRNA secondary structures showing the MFE positional and centroid positional entropy, respectively, for the wild type, 281L/52/440/443 mutant, and 318Ter/12/52 mutant. (c) Minimum free energy (MFE) and centroid secondary structure MFE values. mRNA secondary structures values with 1 kcal/mol above the minimum MFE observed in wild type are considered responsible for the destabilisation of the structure [48]. (d) Positional entropies for the wild type, 281L/52/440/443 mutant, and 318Ter/12/52 mutant. Locations of the variations are indicated. 281L/52/440/443 mutant variations are indicated with red colour. 318Ter/12/52 mutant variations are indicated with green colour. 52 variation which is common in the two mutants is indicated with black colour.

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