Research Article
Activities of Amphioxus GH-Like Protein in Osmoregulation: Insight into Origin of Vertebrate GH Family
Table 1
Sequences of the primers used in this study.
| | Gene | Primer | Sequence (5′ to 3′) | Sequence information |
| | Zebrafish prl | P1 (sense) | GAAACCTGTTCTAGTAATGGCTCAAG | cDNA primer | | P2 (antisense) | CTGCGGTCAGAACTTACCTTAGAAT | | P3 (sense) | CCGGAATTCGTGGGTCTGAATGATTTG | Recombinant primer | | P4 (antisense) | CCGCTCGAGCTAGCACATGTCAGGCC | | Amphioxus nka | P5 (sense) | GCTGGCTACAGTGACGGTATGTC | cDNA primer | | P6 (antisense) | CCAGTTCCAGGTAGGCGTTGT | | P7 (sense) | TGCGTAACTTGGAGGCTGTGGAAA | Real-time PCR primer | | P8 (antisense) | GCCAGGTTGGGTTGCTCTTGTCATA | | Amphioxus nkcc | P9 (sense) | CGCCATCGCTCCTCTCATCTCT | cDNA primer | | P10 (antisense) | CTCGGTAGGTCACTTGCTATTGTCAG | | P11 (sense) | TGACTGGGATGGGTAAGATGAGGC | Real-time PCR primer | | P12 (antisense) | TGGTCATTCTGTCTTTCTTGGTCTCG | | Amphioxus ef1α | P13 (sense) | TGCTGATTGTGGCTGCTGGTACTG | Real-time PCR primer | | P14 (antisense) | GGTGTAGGCCAGCAGGGCGTG | | Amphioxus gh/prllbp | P15 (sense) | GAAGACTCGGACCTGGAGACGCACTA | Real-time PCR primer | | P16 (antisense) | CGTGTTCAGGTAGGCGTGGTCGTA | | Amphioxus igfl | P17 (sense) | CTCATCCGCCCATCAGTA | Real-time PCR primer | | P18 (antisense) | GGTTCTTTCTTGTCCGTTT | | Amphioxus ghl | P19 (sense) | CGCTGTTCTTAGACGAGGTTTTGCT | Real-time PCR primer | | P20 (antisense) | CGGTGATGTCAGTAGGCTGGGTG |
|
|
