Simulated Cholinergic Reinnervation of β (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing β Cell and Cholinergic Cell

Figure 2

INS-1 medium supplemented with HT is the ideal medium for short-term coculture. (a, b, and c) Cellular viability was determined by CCK-8 kits and the data were expressed as percentages of untreated control cells. Results are means ± SD of four independent experiments. (a) INS-1 cells were cultured in INS-1 medium (control) with or without HT or HAT. and and ns versus INS-1 medium group, . (b) NG108-15 cells were cultured in DMEM medium with or without HT or HAT. Cellular viability of NG108-15 cells was determined after 24-hour culture. and ns versus DMEM + HAT group, . (c) NG108-15 cells were cultured in DMEM medium with HAT, INS-1 medium with HT, and INS-1 medium (25 mM Glucose) with HT, respectively. and and ns versus DMEM medium + HAT group, .

(a)

(b)

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