CTLA-4 and PD-1 Ligand Gene Expression in Epithelial Thyroid Cancers

Table 1

Sequence, exon position, and amplicon size of the primers used in quantification of human PD-1 and CTLA-4 gene expression in thyroid carcinomas.

Gene	Primer sequence	Exon	Amplicon size (bp)
PD-L1	5-TATGCCTTGGTGTAGCACTGAC-3	5	139
	5-CCAATGCTGGATTACGTCTC-3	7
PD-L2	5-TTTCATAGCCACAGTGATAGCC-3	5	134
	5-TCCCAAGACCACAGGTTCAG-3	7
CD80	5-CCTGGCTGAAGTGACGTTATC-3	3	143
	5-TTTCCAACCAGGAGAGGTGAG-3	4
CD86	5-CAACACAATGGAGAGGGAAGAG-3	6	89
	5-AAACACGCTGGGCTTCATC-3	7