Prolactin Is a Strong Candidate for the Regulation of Luteal Steroidogenesis in Vizcachas (Lagostomus maximus)

Figure 1

Luteal levels of PRLR, LHR, and 3ß-HSD increased in pregnant-ovulating vizcachas. Representative photomicrographs of luteal cells in ovary cross sections immunostained for PRLR, LHR, and 3ß-HSD, at early pregnancy (EP), midpregnancy (MP), and term pregnancy (TP) of vizcachas. Immunoreactivity is shown in brown and nuclei counterstained with hematoxylin. Immunohistochemical scoring was determined using a 4-point scale: “+” = weak, “++” = moderate, “+++” = strong, and “−” = negative reactivity. LLC: large luteal cells; SLC: small luteal cells; O: nonovulated oocyte into a secondary corpus luteum. Scale bar represents 25 μm.