AMPK Activation Mediated by Hinokitiol Inhibits Adipogenic Differentiation of Mesenchymal Stem Cells through Autophagy Flux

Figure 1

Effect of hinokitiol against adipocyte differentiation in mesenchymal stem cells. MSCs were incubated with hinokitiol at various concentrations (2.5, 5, and 10 μM) following treatment with MDI. The AdipoRed assays were performed on day 6 and were photographed with a light microscope (×200). Fluorescence was measured with an excitation wavelength of 485 nm and an emission wavelength of 572 nm (a and b). Triglyceride (TG) assay (c) and adipolysis (d) were assessed on day 7, and TG contents relative to the control were measured. Total RNA was extracted to quantify the mRNA expression levels of PPAR-γ (e) and C/EBP-α (f). PPAR-γ and C/EBP-α proteins were detected by Western blot analysis (g). β-Actin was used as loading control. A bar graph was generated using mean ± standard error of the mean (SEM) (). and for significant differences between the control and treatment groups.

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