Beta-Arrestin 1 Mediates Liver Thyrotropin Regulation of Cholesterol Conversion Metabolism via the Akt-Dependent Pathway

Figure 3

Immunoblotting analysis of cholesterol metabolism-related proteins in β-arrestin-intervened animals and cells. (a) arrb1/2-KO mice and wild-type littermates were cultivated to 12 weeks, mice were sacrificed, and protein samples of liver tissues were extracted for Western blot using the indicated antibodies. (b) HepG2-Cas9 cells and ARRB1/2-knockdown HepG2-Cas9 cells were cultured to 70–80% confluence, and protein samples were extracted and analyzed by Western blot. All experiments were repeated 3 times with similar results. (c) Grayscale analysis of relative expression level of ARRBs, HMGCR, and CYP7A1 of arrb1/2-KO mice and wild-type littermates. (d) Grayscale analysis of relative expression level of ARRBs, HMGCR, and CYP7A1 of HepG2-Cas9 cells and ARRB1/2-knockdown HepG2-Cas9 cells. . Error bars are calculated as a standard error (SEM).

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