Metformin Regulating miR-34a Pathway to Inhibit Egr1 in Rat Mesangial Cells Cultured with High Glucose

Figure 5

miR-34a inhibitor significantly suppresses miR-34a expression (by quantitative real-time PCR) stimulated by high glucose (a). And miR-34a inhibitor activates SIRT1/AMPKα and restrains Egr1 expression (by western blotting) in high glucose conditions (b) in RMCs. RMCs were transfected with miR-34a inhibitor (IN) or nonspecific control (N-NC) for 6 hours and further cultured with normal glucose (NG, 5.5 mmol/L glucose) or high glucose (HG, 30 mmol/L glucose) media for 24 hours. , versus RMCs cultured with normal glucose media. ^# versus RMCs cultured with high glucose media. All results represent means ± SD obtained from three independent experiments in triplicate. And western blotting results were normalized to GAPDH. SIRT1: sirtuin 1; Egr1: early growth response factor 1; p-AMPKα: phosphorylated adenosine monophosphate-activated protein kinase α; AMPKα: adenosine monophosphate-activated protein kinase α; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.

(a)

(b)