Investigating the Interactive Effects of Sex Steroid Hormones and Brain-Derived Neurotrophic Factor during Adolescence on Hippocampal NMDA Receptor Expression

Figure 2

Protein expression of TrkB, pTrkB, and pERK1/2 in the dorsal (DHP) hippocampus of female sham ovariectomized + placebo (intact), ovariectomized + placebo (OVX), OVX + estradiol (E2), and OVX + progesterone- (P4-) treated mice. (a) Full-length TrkB in the DHP, (b) phosphorylated TrkB (Tyr515) in the DHP, (c) representative blots of TrkB and pTrkB, (d) phosphorylated ERK1 in the DHP, (e) phosphorylated ERK2 in the DHP, and (f) representative blots of pERK1 and 2 and total ERK1 and 2.  = 5–7 per treatment group. ^{^}, , , and . Error bars represent the standard error of the mean (SEM). Two-way ANOVA was performed. Bars with connecting horizontal lines show significant interactions (OVX × genotype), flat bars represent significant main effects of genotype, and above the OVX bar represents post hoc significant differences between OVX and sham control.

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