Investigating the Interactive Effects of Sex Steroid Hormones and Brain-Derived Neurotrophic Factor during Adolescence on Hippocampal NMDA Receptor Expression

Figure 3

Protein expression of NMDA receptors in the dorsal (DHP) and ventral (VHP) hippocampus of male sham castrated + placebo (intact), castrated + placebo (CAST), CAST + testosterone (T), and CAST + dihydrotestosterone- (DHT-) treated mice. (a) GluN1 expression in the DHP, (b) GluN1 expression in the VHP, (c) GluN2A expression in the DHP, (d) GluN2A expression in the VHP, (e) GluN2B expression in the DHP, (f) GluN2B expression in the VHP. Representative blots for each NMDA receptor subtype as well as the loading control.  = 5–7 per treatment group. ^{^}. Error bars represent the standard error of the mean (SEM). Two-way ANOVA was performed. ^ above the CAST + hormone-treated bars represents post hoc trend showing a difference between CAST + hormone-treated groups and CAST placebo.