Glibenclamide-Induced Autophagy Inhibits Its Insulin Secretion-Improving Function in β Cells

Figure 2

Glibenclamide induced autophagy in MIN-6 cells through the AMPK pathway. (a) Cell immunofluorescence analysis. MIN-6 cells were cultured with or without 10 μM glibenclamide in 1640 medium and stained with an anti-LC3 antibody and then observed using a laser confocal microscope. Scale bar: 5 μm. (b) Statistical results of the cell immunofluorescence test. , 1640 vs. 1640 + Glib10. Data shown are mean ± SEM of values from at least 30 cells with triplicate samples. (c–f) Western blot analysis and statistical results of protein quantification. MIN-6 cells were immunoblotted with different antibodies against (c) LC3 and GAPDH; (d) P-S6K and S6K; (e) P-mTOR and m-TOR; and (f) P-AMPK and AMPK.

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