Comparative Proteomic Analysis of Visceral Adipose Tissue in Morbidly Obese and Normal Weight Chinese Women

Figure 1

Workflow of the label-free 1D-LC/MS/MS proteomic strategy. In this work, visceral adipose tissue samples of 9 morbidly obese Chinese women and samples of 8 normal weight controls were extracted, pooled, and digested. The peptides were then sequenced using 1D-LC/MS/MS and label-free proteomic analysis. Technical replicates of mass spectrometry measures were conducted three times. GO analysis and IPA were used to analyze biological functions and pathways. Three candidate proteins were selected and further validated by western blotting analysis. Morphometry and immunohistochemical analyses were conducted between the two groups. Abbreviations: GO, Gene Ontology. IPA, Ingenuity Pathway Analysis.